Journal: Cell Reports Medicine

Article Title: BNT162b2 vaccination effectively prevents the rapid rise of SARS-CoV-2 variant B.1.1.7 in high-risk populations in Israel

doi: 10.1016/j.xcrm.2021.100264

Figure Lengend Snippet:

Article Snippet: Data were calculated using GraphPad Prism 9 for macOS, version 9.0.1(128).

Techniques: Recombinant, Multiplex Assay, Control, Isolation, Lysis, Magnetic Beads, Software, Real-time Polymerase Chain Reaction

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

doi: 10.3389/fbioe.2019.00321

Figure Lengend Snippet: Cell proliferation capacity in human IPFSCs after FN1-KO. FN1-KO cells were compared with copGFP in cell increase (A) , percentage of cells in the S and G 2 phases (B) , and surface markers [SSEA4 (C) , CD73 (D) , CD90 (E) , CD105 (F) , and CD146 (G) ] by flow cytometry; stemness genes ( NANOG, SOX2, KLF4, BMI1, MYC, NOV, POU5F1 , and NES ) (H) , senescent genes ( CDKN1A, CDKN2A , and TP53 ) (I) , and the mesenchymal condensation gene ( CDH2 ) (J) by qPCR. GAPDH was used as an endogenous control. Data are shown as bar charts. * indicates a significant difference compared to the corresponding copGFP group ( P < 0.05).

Article Snippet: The following primary antibodies were used: CD73-APC (cat no. 17-0739-42; eBioScience, Fisher Scientific, Waltham, MA), CD90-APC-Vio770 (cat no. 130-114-863; Miltenyi Biotec, San Diego, CA), CD105-PerCP-Vio700 (cat no. 130-112-170; Miltenyi Biotec), CD146-PE (cat no. 12-1469-42; eBioScience), and the stage-specific embryonic antigen 4-PE (SSEA4-PE; cat no. 330406; BioLegend, Dedham, MA).

Techniques: Flow Cytometry, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

doi: 10.3389/fbioe.2019.00321

Figure Lengend Snippet: Proliferation capacity of human IPFSCs after expansion on the dECMs deposited by FN1-KO cells. Passage 15 human IPFSCs were compared after expansion on dECMs deposited by Cas9-sgFN1a/b transduced cells (sgFN1a ECM and sgFN1b ECM, respectively) with those deposited by copGFP (copGFP ECM) and those grown on TCP (TCP) as controls in cell increase (A) , percentage of cells in the S and G 2 phases (B) , and surface markers [SSEA4 (C) , CD73 (D) , CD90 (E) , and CD105 (F) ] by flow cytometry; stemness genes ( NANOG, SOX2, KLF4, BMI1, MYC, NOV, POU5F1 , and NES ) (G) , senescence genes ( CDKN1A, CDKN2A , and TP53 ) (H) , and the mesenchymal condensation gene ( CDH2 ) (I) by qPCR. GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P < 0.05).

Article Snippet: The following primary antibodies were used: CD73-APC (cat no. 17-0739-42; eBioScience, Fisher Scientific, Waltham, MA), CD90-APC-Vio770 (cat no. 130-114-863; Miltenyi Biotec, San Diego, CA), CD105-PerCP-Vio700 (cat no. 130-112-170; Miltenyi Biotec), CD146-PE (cat no. 12-1469-42; eBioScience), and the stage-specific embryonic antigen 4-PE (SSEA4-PE; cat no. 330406; BioLegend, Dedham, MA).

Techniques: Flow Cytometry, Control

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Frequency of Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and in healthy subjects. ( A ) Distribution of percentages of total lymphocytes, T (CD3+), Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients, compared to healthy subjects. Shown is mean ± SD. ( B ) Representative dot-plots showing the frequency of Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and healthy subjects. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques:

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Phenotypic analysis of circulating Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and in healthy subjects. Distribution of ex vivo memory subsets of Vδ1+ ( A ) and Vδ2+ ( C ) T lymphocytes based on the expression of CD27 and CD45RA in hospitalized and recovered COVID-19 patients, compared to healthy donors. Median is shown. Healthy donors were represented as triangle, COVID-19 patients as circle and COVID-19 recovered as rhombus. Representative dot-plots showing Vδ1+ ( B ) and Vδ2+ ( D ) T lymphocyte memory subset distribution in hospitalized, recovered, and healthy subjects. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques: Ex Vivo, Expressing

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Analysis of exhaustion-marker expression by circulating Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and healthy subjects. Expression of TIM-3 and PD-1 on Vδ1+ ( A ) and Vδ2+ ( B ) T lymphocytes in hospitalized and recovered COVID-19 patients, compared to healthy donors. Shown are percentage ( left panels ) and MFI ( right panels ) ± SD. ( C ) Representative dot-plots of exhaustion-marker expression by Vδ1+ and Vδ2+ T lymphocytes from hospitalized and recovered COVID-19 patients, compared to healthy donors. ( D ) Frequency of exhausted Vδ1+ and Vδ2+ T cells per 1 × 10 6 T lymphocytes. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques: Marker, Expressing

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Expression of pro-inflammatory cytokines by Vδ1+ T lymphocytes in hospitalized and recovered COVID-19 patients, compared to healthy subjects. Cumulative histograms representing the expression of pro-inflammatory cytokines by Vδ1+ T lymphocyte. Shown are percentage ( left panels ), MFI ( central panel , log 10 scale), and iMFI ( right panels ) ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques: Expressing